Elucidating the mechanism of cellular uptake and removal

Our results show that NP uptake and transport are dependent on the tumor cell type.

MDA-MB-231 tissue showed deeper penetration of GNPs as compared to MCF-7 one.

Burlington, ON) with 10 % Fetal Bovine Serum (Sigma-Aldrich, Oakville, ON). The thickness of the tissue was controlled by the growth period.

MCL incubation with NPs was done by hanging the MCLs in multiwall plates followed by filling the top of the inserts with the GNP and media mixture.

Tokyo, Japan), UV-spectroscopy (Lambda 40; Perkin Elmer, Waltham, MA), and by dynamic light scattering using 90 Plus Particle Sizer Analyzer (Brookhaven Instruments Corp.

New York, NY) to determine the size of the particles. After reaching confluence, these cells were trypsinized, centrifuged, suspended in media, and counted.

In this study, 20-nm particles were chosen since our future goal is to use these NPs for gene delivery.

The interface between tumor vasculature and tumor tissue is highlighted with a .Intracellular and extracellular distributions of NPs were mapped using Cyto Viva imaging.The ability of MCLs to mimic tumor tissue characteristics makes them a useful tool in assessing the efficacy of particle distribution in solid tumors.].Since the proliferation of tumor cells can outpace the proliferation of cells that form blood vessels, vascular density can be reduced, and the existence of cells as far as 100 µm from blood vessels is possible [].Hence, our MCL model creates a reasonable tumor microenvironment to study NP transport in tumor tissue.

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With pore sizes of 3 µm, the inserts allowed for the passage of stirred media through the base of the insert as seen in Fig. Two breast cancer cell lines were used in this study: MCF-7 and MDA-MB-231.

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